Therefore, make sure that you do not touch the bulb and only move the lamp via it's base. Coloured filters can be used, but these will affect light intensity by different amounts depending on the colour. The number of light photons hitting the chlorophyll pigments increase as the light intensity rises. Dehydration may even damage the micromolecular structure of the chloroplasts. Internal Factors 1 chlorophyll —It is the most important internal factor for photosynthesis.
Results Table 1 Environmental condition Number of Oxygen bubbles Trial 1 Trial 2 Trial 3 Average Lamp 5cm from plant 83 77 89 83 Lamp 20cm from plant 28 21 19 23 Plant in sodium bicarbonate; lamp 5cm 99 109 113 107 DiscussionLight intensity affects photosynthesis. Oxygen is the waste product that is given out via the stomata pores when they become turgid, this is during the process of respiration. As the carbon dioxide in the water has decreased, the acidity of the water decreases causing the indicator to change from red to purple. Alhtough I am quite puzzled myself at why there was sometimes a decrease in percentage increase, even through extentive research I have been unable to find the answer. Suppose a leaf is subjected to a light intensity sufficient to decompose 5 c. Standards Independent variable Time is the independent variable and will be measured with a stop watch. If they reflect it, then they're not absorbing it, right? They have different sizes and distributions of the intercellular spaces.
At a very high oxygen concentration, the rate of photosynthesis begins to decline in all the plants. Light intensity directly affects the light-dependent reaction in photosynthesis and indirectly affects the light-independent reaction. Thanks for asking, Answer 2: So by level of light you probably mean light intensity which is something that can be measured. When you think the water colour has changed to that shade of purple, you then record the results. Most pigments don't absorb ultraviolet light well, and if the wavelength is too low they can become damaging to plant cells. When temperature increases from minimum to optimum, the rate of photosynthesis doubles for every 10°C rise in temperature. .
The equipment list is extremely easy to do. Yes, another boring which makes it vital to have no slip ups in the method, validity, reliability, evaluation or conclusion. To do this, the elodea must be completely be submerged in deionized water, later place the two plants under artificial light and natural light. The rate of photosynthesis will be measured by the number of bubbles given off considering that oxygen is produced as a waste gas during photosynthesis. Maybe it's already been said, or maybe it's obvious - but too high light intensities have a damaging effect - some are perfectly happy to hang around in the gloom under the sea. This is only a demonstration of working method which can be refined and used for a full scale science project.
However it is probably a good idea to replace it every so often with a fresh supply from an aquarist centre or a pond. Research Question and Hypothesis Does light intensity affect the rate of photosynthesis in Elodea plants? Chlorophyll is the green substance found in chloroplasts and this is why leaves look green. You might get a question literally asking for what appartus you used in the exam: it varies. Duration of Light Photosynthesis can occur in continuous illumination without any harm to the plant. My theory behind this is the following; Photosynthesis is the process that plants use to create energy for themselves.
This method involves usage of flammable, volatile and toxic substances. Materials and Methods The procedure as set out in the Molecules, Genes and Cells Laboratory Manual 2016 was followed. Sugar is also converted into starch and the accumulation of starch in chloroplasts reduces their effective surfaces and the process slows down. Cyanobacteria Cyanobacteria, also known as blue-green algae, are responsible for a large portion of the photosynthesis that takes place in the ocean. Set up your meter ruler and lamp 50 cm away from the Elodea. Light energy is trapped by chlorophyll.
We can start the experiment having the light source further away from the pondweed. Plants do not survive below the light compensation point. According to this law the rate of chemical reaction doubles for every rise of 10°C. Distilled water is one of the cleanest types of water. At 27 mm away from the light, the absorbance decreased at 0. There are two views about the affect of chlorophyll on photosynthesis: i Willstatter and Stoll: They believe that the rate of photosynthesis is not proportional to the amount of chlorophyll content.
If the temperature is too high, the enzyme becomes denatured and it stops photosynthesis. Situated in the membrane of the thylakoid membrane is the electron transport chain. Light: The photosynthetically active region of the spectrum of light is at wavelengths from 400-700 nm. The set-up was left alone for two minutes to stabilise. We can do the same for temperature. Abstract: The overall aim of this report was the conduct an experiment based how different light intensity levels, representing the four seasons in a year: summer, spring, autumn and winter, affects the transpiration rate in a mangrove plant.
Move the lamp so it is 20 cm from the plant, after several minutes count bubbles and record. Photophilous plants responds favourably to higher light intensities than sciophilous plants. Much of this is oxidized by living organisms for survival. Answer 3: This is a very important aspect of photosynthesis. In nature, balance is crucial. From further away, the light intensity will be less, and therefore light will be a limiting factor, however when we increase the light intensity by moving the source of light closer towards the Elodea, which then means that light cannot be the limiting factor of photosynthesis. Repeat the count and calculate the mean rate of bubble production as before.
There is a slight risk of infection from pond water, so take sensible hygiene precautions, cover cuts and wash hands thoroughly after the work is complete. In one swift movement, the test tube with the sodium bi-carbonate and distilled water solution in it was flipped upside down on top of the thin part of the funnel. Carbon Dioxide Increase in carbon dioxide increases the rate of photosynthesis in most C3 plants. In this example the intensity of light is the limiting factor for the rate of photosynthesis. It affects everything in the biological chain, either directly or indirectly. Clearly, the chlorophyll in the green leaves is surplus. However they have different property called polarity.